Title: Environmental stress and the clock in Drosophila
Host Institution: Jagiellonian University (Krakow)
Supervisor: Prof Elzbieta Pyza
Start date: 1st September 2018
Secondment: University of Padova for 4 months for generating molecular constructs for mutagenesis.
Objectives: To investigate the role of neuronal and glial Heme oxygenase (HO) on the circadian clock and associated phenotypes after stress. HO plays a role in the DNA damage signalling pathway and shows two peaks of expression during the day that are regulated by the clock. Furthermore changes in HO expression alter circadian behaviour. We shall examine its expression patterns in the brain, particularly synaptic plasticity, under various environmental stresses (hot, cold, pesticide) and asses the DNA damage response in wild type and CRISPR/Cas9 generated ho mutants. We shall also use antioxidants to see if they have beneficial effects under normal and stress conditions.
About me: I am from Nepal, a beautiful Himalayan nation. I received my MS degree in Pharmacy from Inje University, South Korea. From the very beginning of my undergraduate days, I saw my interest tilted towards research and innovation, which drove me to choose MS degree by research in South Korea where I received ample research opportunities. The research in my MS degree greatly ignited my interest towards Drosophila model with a great interest in chronobiology.
As a part of CINCHRON, I would be working under the supervision of Prof Elzbieta Pyza to determine the role of glial Heme oxygenase (HO) on the circadian clock and associated phenotypes after stress. I believe I can collaborate with different groups within the CINCHRON to take my level of knowledge regarding Chronobiology to a new level and contribute as a team and individually to CINCHRON that might ascertain innovative findings.